mouse antihuman cd106  (SouthernBiotech)

Journal:

Article Title: Normal human IgG prevents endothelial cell activation induced by TNF? and oxidized low-density lipoprotein atherogenic stimuli

doi: 10.1046/j.1365-2249.2003.02215.x

Figure Lengend Snippet: (A–D) Effect of IgG on TNFα and oxLDL-dependent CD106 expression by EC. (A) EC incubated with 1 nmol/l TNFα for 4 h exposed CD106 as shown by direct immunofluorescence with a monoclonal antihuman CD106. (B) expression of CD106 was entirely inhibited in EC pre-incubated with 0·05 mmol/l IVIg for 24 h and washed before TNFα incubation. (C) immunofluorescence membrane labelling of CD106 after 4 h incubation with oxLDL 40 µg/ml. (D) pre-incubation with 0·05 mmol/l IVIg for 24 h completely prevented CD106 expression. Scale bar = 5 µm. (E–F) Effect of IgG on oxLDL internalization by EC. Intracellular signal was detectable by CLSM after about 10 minutes incubation of EC with 40 µg/ml oxLDL-FITC. (E) the fluorescence pattern after 40 min incubation includes filaments and granules (arrowheads: granules). (F) pretreatment with 0·05 mmol/l IVIg for 24 h strongly reduces internalization of oxLDL, in particular filaments are not detectable. This image was acquired after 40 min incubation with oxLDL. Scale bar = 10 µm. The colour scale shown in panel A applies to all colour images.

Article Snippet: After washing, cells were incubated with a mouse monoclonal antihuman CD106 (Serotec Ltd, Oxford, UK), washed and the reaction revealed with an antimouse antibody coupled to fluorescein.

Techniques: Expressing, Incubation, Immunofluorescence, Fluorescence